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htert promoter  (Addgene inc)


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    Structured Review

    Addgene inc htert promoter
    Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with <t>hTERT</t> promoter (shown in purple) replacing the <t>native</t> <t>E1A</t> promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.
    Htert Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert promoter/product/Addgene inc
    Average 91 stars, based on 2 article reviews
    htert promoter - by Bioz Stars, 2026-02
    91/100 stars

    Images

    1) Product Images from "Development of Oncolytic Vectors Based on Human Adenovirus Type 6 for Cancer Treatment."

    Article Title: Development of Oncolytic Vectors Based on Human Adenovirus Type 6 for Cancer Treatment.

    Journal: Viruses

    doi: 10.3390/v15010182

    Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with hTERT promoter (shown in purple) replacing the native E1A promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.
    Figure Legend Snippet: Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with hTERT promoter (shown in purple) replacing the native E1A promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.

    Techniques Used: Recombinant, Virus, Plasmid Preparation



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    Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with <t>hTERT</t> promoter (shown in purple) replacing the <t>native</t> <t>E1A</t> promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.
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    Image Search Results


    Ad35, adenovirus serotype 35; BGHpA, bovine growth hormone polyadenylation signal; CMV, cytomegalovirus immediate-early enhancer and promoter; EGFP, enhanced green fluorescence protein gene; hTERT, human telomerase reverse transcriptase promoter; ITR, inverted terminal repeat; IRES, internal ribosomal entry site; native, native E1B gene promoter; SV40pA, the simian virus 40 polyadenylation signal.

    Journal: PLOS ONE

    Article Title: Novel conditionally replicating adenovirus-mediated efficient detection of circulating tumor cells in lung cancer patients

    doi: 10.1371/journal.pone.0286323

    Figure Lengend Snippet: Ad35, adenovirus serotype 35; BGHpA, bovine growth hormone polyadenylation signal; CMV, cytomegalovirus immediate-early enhancer and promoter; EGFP, enhanced green fluorescence protein gene; hTERT, human telomerase reverse transcriptase promoter; ITR, inverted terminal repeat; IRES, internal ribosomal entry site; native, native E1B gene promoter; SV40pA, the simian virus 40 polyadenylation signal.

    Article Snippet: The hTERT promoter-driven E1 and GFP gene expression cassette in which the P2A peptide-coding sequence and the GFP gene were located downstream of the E1A gene was chemically synthesized (GENEWIZ Japan Corp, Tokyo, Japan) and was inserted into pAdHM34 to obtain pAdHM34-hAGB-miR142T.

    Techniques: Fluorescence, Virus

    Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with hTERT promoter (shown in purple) replacing the native E1A promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.

    Journal: Viruses

    Article Title: Development of Oncolytic Vectors Based on Human Adenovirus Type 6 for Cancer Treatment.

    doi: 10.3390/v15010182

    Figure Lengend Snippet: Figure 1. Construction of Ad6-hT-GM. pShuttle is based on pBR322 and contains the left part of the Ad6 genome from LITR to 1773 bp (shown in yellow) with hTERT promoter (shown in purple) replacing the native E1A promoter and the right part, from 27,576 bp to RITR (shown in green) with the GM-CSF transgene inserted instead of E3-6.7K and E3-gp19K genes (shown in cyan). pShuttle was linearized by amplification with primers containing 15 nucleotide homology arms (shown in orange). The Ad6 genome was digested with ClaI and PacI, and recombination between truncated genome and linearized pShuttle was performed by the In-Fusion kit. Ad6-hT-GM recombinant virus genome was then retrieved from the plasmid with the AsiSI. Expanded regions indicate modified Ad6-hT-GM genome regions.

    Article Snippet: After that, the native E1A promoter (360–559 bp) was substituted by hTERT promoter (from pAdVTERT plasmid #26744, Addgene) using primers TERT-pShuttle-for and TERT-pShuttle-rev (Table S1) for the amplification of hTERT and pShuttle-TERT-for and pShuttle-TERT-rev (Table S1) for the amplification of pShuttle resulting in pShuttle-hTE1A-AdITRs.

    Techniques: Recombinant, Virus, Plasmid Preparation